Human IgG antibody Laboratories manufactures the intra op pth assay reagents distributed by Genprice. The Intra Op Pth Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact pth assay. Other Intra products are available in stock. Specificity: Intra Category: Op Group: Pth Assay
Pth Assay information
Parathyroid Hormone (PTH) Magnetic Luminex Assay Kit |
LKU606991-96T |
Biomatik Corporation |
96T |
EUR 944.2 |
ChoosE2-Opti™ Intrachain TR-FRET Assay Kit |
78561 |
BPS Bioscience |
384 reactions |
EUR 2150 |
Description: ChoosE2-Opti™ Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to identify E2 enzyme(s) that are functional partners for a purified E3 ubiquitin ligase of interest and lead to its self-polyubiquitination. The assay kit contains five E2 enzymes to be tested with the E3 ligase of interest, and two E3 ligases (SMURF1 and MDM2) to use as internal controls. It also contains an E1 enzyme, ATP, an optimized TRF Ubiquitin Mix, and a universal buffer. It utilizes a Europium-labeled Ubiquitin Donor as well as Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into polyubiquitin chains, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses. |
Anti-Can Intra-Acrosomal Protein Purified |
11-281-C025 |
ExBio |
0.025 mg |
EUR 135.6 |
Anti-Can Intra-Acrosomal Protein Purified |
11-281-C100 |
ExBio |
0.1 mg |
EUR 223.2 |
Cell Meterâ„¢ Fluorimetric Intracellular pH Assay Kit |
21180-1000Tests |
AAT Bioquest |
1000 Tests |
EUR 446 |
|
Description: Intracellular pH change are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders and Alzheimer's disease. |
ChoosE3-Freedom™ Intrachain TR-FRET Assay Kit |
78560 |
BPS Bioscience |
384 reactions |
EUR 2150 |
Description: The ChoosE3-Freedom Intrachain TR-FRET Assay Kit contains E1 and E2 enzymes, ATP, an optimized TRF Ubiquitin Mix, and a universal buffer. Purified E3 ligase MDM2 is also provided as an internal quality control. The assay was designed with a Europium-labeled Ubiquitin donor and a Cy5-labeled Ubiquitin acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on the E3 ligase, the assay measures only poly-ubiquitination and not mono-ubiquitination. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics. |
Cell Meter™ Fluorimetric Intracellular pH Assay Kit |
21180 |
AAT Bioquest |
1000 Tests |
EUR 446 |
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
STA-342 |
Cell Biolabs |
96 assays |
EUR 762 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
STA-342-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3058.8 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
Human Primary Intra-Hepatic Biliary Epithelial Cells |
T4221 |
ABM |
5x10^5 cells / 1.0 ml |
Ask for price |
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
STA-800 |
Cell Biolabs |
96 assays |
EUR 588 |
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
STA-800-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2307.6 |
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size |
STA-342-T |
Cell Biolabs |
20 assays |
EUR 414 |
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
CBL-B-driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit |
78388 |
BPS Bioscience |
384 rxns. |
EUR 2310 |
Description: Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications regulating protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2~Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2~Ub to the substrate, leading to its mono- or poly-ubiquitination.Casitas B-lineage lymphoma proto-oncogene-b (CBL-B) is the RING-type E3 ligase that functions as a negative regulator of T cell activation and of growth factor receptor and non-receptor tyrosine kinase signaling. It contains an N-terminal tyrosine kinase binding (TKB) domain comprised of a four-helix bundle, a calcium binding EF-hand and a Src homology (SH2) domain, followed by a linker helical region and the RING domain, responsible for its catalytic function. Additionally, CBL-B contains proline-rich regions mediating the association with tyrosine- and serine phosphorylation sites, and a ubiquitin-associated (UBA)/leucine zipper domain for dimerization. CBL-B interacts with a large number of target proteins implicated in the control of cell proliferation, differentiation, and cell morphology. The ubiquitin ligase activity of CBL-B is up-regulated by the phosphorylation of Tyrosine (Tyr) 363, which is located in the helix linker between the TKB and RING domains. Phosphorylation of Tyr363 opens CBL-B from its auto-inhibitory confirmation, allowing E2 and substrates to bind to CBL-B. CBL-B is phosphorylated for example by receptor-type tyrosine kinase Tyro3, which also serves as a substrate for CBL-B ubiquitylation both in vitro and in vivo.It utilizes a Europium cryptate-labeled Ub (donor) and a Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetics analyses of polyubiquitination. Of note, the assay kit does not detect mono-ubiquitination. |
C-CBL-driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit |
78408 |
BPS Bioscience |
384 rxns. |
EUR 2150 |
Description: The C-CBL-driven Tyro3 Ubiquitination Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure C-CBL E3 ligase activity in a homogeneous 384 reaction format. It utilizes a Europium cryptate-labeled Ub (donor) and a Cy5-labeled Ub (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput applications as well as real-time analyses of polyubiquitination. Of note, the assay kit does not detect mono-ubiquitination.Alternatively, under the same experimental conditions the AXL kinase (BPS Bioscience #40180) can be used instead of Tyro3 as C-CBL ubiquitination substrate. |
Cell Meterâ„¢ Intracellular GSH Assay Kit *Optimized for Flow Cytometry* |
22810-100Tests |
AAT Bioquest |
100 Tests |
EUR 222 |
|
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. |