Blasticidin Selection Protocol

Cas9 Lentivirus (Puromycin Selection)
78066 500 µl x 2
EUR 795
Description: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene._x000D_
Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines._x000D_
The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Puromycin selection marker._x000D_

Human IgG antibody Laboratories manufactures the blasticidin selection protocol reagents distributed by Genprice. The Blasticidin Selection Protocol reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Blasticidin products are available in stock. Specificity: Blasticidin Category: Selection Group: Protocol

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration
10 µg
EUR 1071

True north Cryobox 0.2mLNatural
PK10
EUR 172.8

True north Cryobox 0.5mLNatural
PK10
EUR 153.6

True north Cryobox1.5/2mLNatural
PK10
EUR 100.8

True north Cryobox 5mLNatural
PK10
EUR 153.6

True north Cryobox 5mLBlue
PK10
EUR 153.6

True north Cryobox 15mLNatural
PK10
EUR 212.4

Protocol information

shRNA-U6 (Neg)-(blasticidin) lentivirus
U6(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

CRE stable cells with Blasticidin marker
SC004-Bsd 2 x 106 cell/ml x 1ml
EUR 1365.6
Description: CRE recombinase stable cell line with Blasticidin resistance

Blasticidin S HCl Solution (10 mg/mL in 20mM HEPES)
B006-10x1ML 10 x 1 mL
EUR 405.6

Blasticidin S HCl Solution (10 mg/mL in 20mM HEPES)
B006-1ML 1 mL
EUR 72

Blasticidin S HCl Solution (10 mg/mL in 20mM HEPES)
B007-20ML 20 mL
EUR 517.2

Luciferase (firefly), Hela stable cells (Blasticidin)
SC032-Bsd 2 x 106 cell/ml x 1ml
EUR 1452
Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

luciferase (firefly), HEK293 stable cells (Blasticidin)
SC002-Bsd 2 x 106 cell/ml x 1ml
EUR 843.6
Description: Luciferase (firefry) stable cell line with Blasticidin marker.

Luciferase-2A-GFP, Hela stable cells (Blasticidin)
SC032-GB 2 x 106 cell/ml x 1ml
EUR 2148
Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Blasticidin marker.

Luciferase-2A-RFP, Hela stable cells (Blasticidin)
SC032-RB 2 x 106 cell/ml x 1ml
EUR 2148
Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Blasticidin marker.

luciferase (firefly), HEK293 stable cells (RFP, Blasticidin)
SC002-RB 2 x 106 cell/ml x 1ml
EUR 1104
Description: Luciferase (firefry) stable cell line with RFP-Blasticidin dual marker.

EZSolution? Blasticidin S Hydrochloride, Sterile-Filtered
2805-10 each
EUR 588

HEK293-CFTR cell line with RFP and Blasticidin dual marker
SC022-RB 2 x 106 cell/ml x 1ml
EUR 5280
Description: Human CFTR ion-channel stable cell line with RFP-Blasticidin dual marker.

HEK293-CLCN2 cell line with RFP and Blasticidin dual marker
SC023-RB 2 x 106 cell/ml x 1ml
EUR 5280
Description: Human CLCN2 ion-channel stable cell line with RFP-Blasticidin dual marker.

AAV-1 Helper Free Bicistronic Expression System (Blasticidin)
VPK-419-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Bicistronic Expression System (Blasticidin)
VPK-419-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Bicistronic Expression System (Blasticidin)
VPK-419-SER3 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Bicistronic Expression System (Blasticidin)
VPK-419-SER4 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).