Blasticidin Or Puromycin

Puromycin

G264 1.0 ml; 10 mg/ml
EUR 60
Description: Extracted from Streptomyces alboniger bacteria, Puromycin is an aminonucleoside antibiotic that inhibits protein synthesis by interfering with the translation process. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Fungi and Gram negative bacteria are resistant to Puromycin due to its low permeability. But in some particular conditions puromycin can be used for E. coli. For more than 30 years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harbouring plasmids carrying puromycin resistance genes. ;RESISTANCE TO PUROMYCIN;

The pac gene encoding a Puromycin N-acetyl-transferase (PAC) has been isolated from a Streptomyces productin strain1,2. It is located in a region of the pur cluster linked to the other genes determining the puromycin biosynthetic pathway. The expression of pac gene confers puromycin resistance to transfected mammalian cells3 expressing it. Under some conditions puromycin could also be used for selection of E. coli strains transformed with plasmids carrying the pac gene.

CONDITIONS OF SELECTION

Puromycin is poorly active on E. coli but is particularly useful in experiments conducted with mammalian cells. It can be used as an alternative to the Neomycin system for transfection experiments.

Mammalian cells

The working concentrations of puromycin for mammalian cell lines range from 1 to 10µg/mL. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach form the plates allowing easy and early identification of transformant clones.

References:

1) VARA J., et al. (1985). Biochemistry. 24: 8074-8081

2) LACALLE R.A., et al. (1989). Gene. 79: 375-380

3) DE LA LUNA S and J. ORTIN. (1992). Methods In Enzymology. 216: 376-385

Human IgG antibody Laboratories manufactures the blasticidin or puromycin reagents distributed by Genprice. The Blasticidin Or Puromycin reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Blasticidin products are available in stock. Specificity: Blasticidin Category: Or Group: Puromycin

Puromycin

1mg Ask for price
Description: Puromycin

Puromycin

50mg Ask for price
Description: Puromycin

Puromycin

5mg Ask for price
Description: Puromycin

Puromycin

100 mg
EUR 452.4

Puromycin

25 mg
EUR 250.57
Description: C22H29N7O5

Puromycin

5 mg
EUR 71.53
Description: C22H29N7O5

JBS True Blue

300µl
EUR 13.7

Puromycin information

Blasticidin S Hydrochloride

B001-1G 1 g
EUR 1146.4
Description: C17H26N8O5 • HCl

Blasticidin S Hydrochloride

B001-25MG 25 mg
EUR 70.81
Description: C17H26N8O5 • HCl

Blasticidin S Hydrochloride

B001-2x25MG 2 x 25 mg
EUR 186

Blasticidin S hydrochloride

HY-103401 25mg
EUR 469.2

50mg Blasticidin S HCl - EACH

30-100-RB EACH
EUR 260.55

A549 / GFP stable cells (Blasticidin)

SC043-Bsd 2 x 106 cell/ml x 1ml
EUR 930
Description: GFP expression stable cells in A549 human lung cancer cell line with Blasticidin resistance

CHO-GFP stable cells (Blasticidin)

SC039-Bsd 2 x 106 cell/ml x 1ml
EUR 930
Description: GFP expression stable cells in CHO cell line with Blasticidin resistance

Hela-GFP stable cells (Blasticidin)

SC034-Bsd 2 x 106 cell/ml x 1ml
EUR 930
Description: GFP expression stable cell line in Hela cells with Blasticidin marker.

RKO / GFP Stable Cell Line (Blasticidin)

SC047-GB 2 x 106 cell/ml x 1ml
EUR 1452
Description: GFP expression stable cells in RKO Cells, human colorectal carcinoma cells with Blasticidin resistance

shRNA-H1 (Neg)-(blasticidin) lentivirus

H1(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

shRNA-U6 (Neg)-(blasticidin) lentivirus

U6(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

CRE stable cells with Blasticidin marker

SC004-Bsd 2 x 106 cell/ml x 1ml
EUR 1365.6
Description: CRE recombinase stable cell line with Blasticidin resistance

Blasticidin S HCl Solution, 10mg/mL, Sterile

40110070-1 1 mL
EUR 77.81

Blasticidin S HCl Solution, 10mg/mL, Sterile

40110070-2 10 mL
EUR 515.16

HEK293-TRPV1 with RFP and Blasticidin marker

S0011003 One Frozen vial
EUR 2600

Luciferase (firefly), Hela stable cells (Blasticidin)

SC032-Bsd 2 x 106 cell/ml x 1ml
EUR 1452
Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

Blasticidin S HCl Solution (10 mg/mL in 20mM HEPES)

B006-10x1ML 10 x 1 mL
EUR 266.37
Description: C17H26N8O5 • HCl