Blasticidin Or Puromycin
Puromycin |
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G264 | ABM | 1.0 ml; 10 mg/ml | EUR 60 |
Description: Extracted from Streptomyces alboniger bacteria, Puromycin is an aminonucleoside antibiotic that inhibits protein synthesis by interfering with the translation process. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Fungi and Gram negative bacteria are resistant to Puromycin due to its low permeability. But in some particular conditions puromycin can be used for E. coli. For more than 30 years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harbouring plasmids carrying puromycin resistance genes. ;RESISTANCE TO PUROMYCIN; The pac gene encoding a Puromycin N-acetyl-transferase (PAC) has been isolated from a Streptomyces productin strain1,2. It is located in a region of the pur cluster linked to the other genes determining the puromycin biosynthetic pathway. The expression of pac gene confers puromycin resistance to transfected mammalian cells3 expressing it. Under some conditions puromycin could also be used for selection of E. coli strains transformed with plasmids carrying the pac gene. CONDITIONS OF SELECTION Puromycin is poorly active on E. coli but is particularly useful in experiments conducted with mammalian cells. It can be used as an alternative to the Neomycin system for transfection experiments. Mammalian cells The working concentrations of puromycin for mammalian cell lines range from 1 to 10µg/mL. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach form the plates allowing easy and early identification of transformant clones. References: 1) VARA J., et al. (1985). Biochemistry. 24: 8074-8081 2) LACALLE R.A., et al. (1989). Gene. 79: 375-380 3) DE LA LUNA S and J. ORTIN. (1992). Methods In Enzymology. 216: 376-385 |
Human IgG antibody Laboratories manufactures the blasticidin or puromycin reagents distributed by Genprice. The Blasticidin Or Puromycin reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Blasticidin products are available in stock. Specificity: Blasticidin Category: Or Group: Puromycin
Puromycin |
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TargetMol Chemicals | 1mg | Ask for price |
Description: Puromycin |
Puromycin |
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TargetMol Chemicals | 50mg | Ask for price |
Description: Puromycin |
Puromycin |
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TargetMol Chemicals | 5mg | Ask for price |
Description: Puromycin |
Puromycin |
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TOKU-E | 100 mg | EUR 452.4 |
Puromycin |
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TOKU-E | 25 mg | EUR 250.57 |
Description: C22H29N7O5 |
Puromycin |
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TOKU-E | 5 mg | EUR 71.53 |
Description: C22H29N7O5 |
JBS True Blue |
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Jena Bioscience GmbH | 300µl | EUR 13.7 |
Blasticidin S Hydrochloride |
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B001-1G | TOKU-E | 1 g | EUR 1146.4 |
Description: C17H26N8O5 • HCl |
Blasticidin S Hydrochloride |
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B001-25MG | TOKU-E | 25 mg | EUR 70.81 |
Description: C17H26N8O5 • HCl |
Blasticidin S Hydrochloride |
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B001-2x25MG | TOKU-E | 2 x 25 mg | EUR 186 |
Blasticidin S hydrochloride |
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HY-103401 | MedChemExpress | 25mg | EUR 469.2 |
50mg Blasticidin S HCl - EACH |
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30-100-RB | Scientific Laboratory Supplies | EACH | EUR 260.55 |
A549 / GFP stable cells (Blasticidin) |
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SC043-Bsd | GenTarget | 2 x 106 cell/ml x 1ml | EUR 930 |
Description: GFP expression stable cells in A549 human lung cancer cell line with Blasticidin resistance |
CHO-GFP stable cells (Blasticidin) |
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SC039-Bsd | GenTarget | 2 x 106 cell/ml x 1ml | EUR 930 |
Description: GFP expression stable cells in CHO cell line with Blasticidin resistance |
Hela-GFP stable cells (Blasticidin) |
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SC034-Bsd | GenTarget | 2 x 106 cell/ml x 1ml | EUR 930 |
Description: GFP expression stable cell line in Hela cells with Blasticidin marker. |
RKO / GFP Stable Cell Line (Blasticidin) |
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SC047-GB | GenTarget | 2 x 106 cell/ml x 1ml | EUR 1452 |
Description: GFP expression stable cells in RKO Cells, human colorectal carcinoma cells with Blasticidin resistance |
shRNA-H1 (Neg)-(blasticidin) lentivirus |
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H1(shRNA-Ctr)-Bsd | GenTarget | 1 x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter. |
shRNA-U6 (Neg)-(blasticidin) lentivirus |
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U6(shRNA-Ctr)-Bsd | GenTarget | 1 x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter. |
CRE stable cells with Blasticidin marker |
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SC004-Bsd | GenTarget | 2 x 106 cell/ml x 1ml | EUR 1365.6 |
Description: CRE recombinase stable cell line with Blasticidin resistance |
Blasticidin S HCl Solution, 10mg/mL, Sterile |
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40110070-1 | Glycomatrix | 1 mL | EUR 77.81 |
Blasticidin S HCl Solution, 10mg/mL, Sterile |
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40110070-2 | Glycomatrix | 10 mL | EUR 515.16 |
HEK293-TRPV1 with RFP and Blasticidin marker |
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S0011003 | Addexbio | One Frozen vial | EUR 2600 |
Luciferase (firefly), Hela stable cells (Blasticidin) |
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SC032-Bsd | GenTarget | 2 x 106 cell/ml x 1ml | EUR 1452 |
Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker. |
Blasticidin S HCl Solution (10 mg/mL in 20mM HEPES) |
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B006-10x1ML | TOKU-E | 10 x 1 mL | EUR 266.37 |
Description: C17H26N8O5 • HCl |